IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
نویسندگان
چکیده
منابع مشابه
IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
Hens can be immunized by means of i.m. vaccination (Musculus pectoralis, left and right, injection volume 0.5-1.0 ml) or by means of Gene-Gun plasmid-immunization. Dependent on the immunogenicity of the antigen, high antibody-titres (up to 1:100,000 - 1:1,000,000) can be achieved after only one or 3 - 4 boost immunizations. Normally, a hen lays eggs continuously for about 72 weeks, thereafter t...
متن کاملidentification and extraction of chicken egg yolk immunoglobulin from egg by polyethylene glycol (peg) precipitation
background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...
متن کاملComparison of Four Different Purification Methods for Isolation of Anti Echis carinatus Antivenom Antibodies from Immunized Chicken Egg Yolk
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...
متن کاملGENERATION AND PHYSICOCHEMICAL CHARACTERIZATION OF CHICKEN EGG YOLK ANTIBODIES (IgY) AGAINST Salmonella typhimurium
Salmonellosis is one of the most important bacterial diseases with economic concern to all phases of the poultry. The study was aimed to generate chicken IgY against Salmonella typhimurium and its physicochemical characterization to unbolt the obstacles for the usage of IgY as therapeutic agent in Poultry. Salmonella typhimurium whole cell antigen was prepared and immunized in white leghorn chi...
متن کاملGeneration of Egg Yolk Antibodies in Chicken ( IgY ) Against Influenza M 2 ( M 2 e ) Protein
Chicken egg yolk immunoglobulins (IgY) from immunized hens have considerable advantages for the production of polyclonal antibodies. It reacts with more epitopes and avoids the interference in immunological assays. Regarding the IgY aptitudes, this study was conducted to produce chicken IgY specific for influenza M2 (M2e) protein. Fusion construct harboring C-terminal of bovine heat shock prote...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3084